| 质粒名称: | pC.HA Vav2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 14554 |
| 存储实验室: | Joan Brugge |
|
载体骨架基本信息 |
|
| 载体名称: | pC.HA |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Vav2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2600 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | HA（骨干网上的C终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XbaI(未破坏) |
| 3' 克隆位点: | SpeI（销毁时未知） |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pC.HA Vav2 was a gift from Joan Brugge (Addgene plasmid # 14554 ; http://n2t.net/addgene:14554 ; RRID:Addgene_14554) |
| 参考文献部分: | Vav family proteins couple to diverse cell surface receptors. Moores SL, Selfors LM, Fredericks J, Breit T, Fujikawa K, Alt FW, Brugge JS, Swat W. Mol Cell Biol. 2000 Sep . 20(17):6364-73. 10.1128/MCB.20.17.6364-6373.2000 PubMed 10938113 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pC.HA Vav2 7666 bp ds-DNA circular SYN 26-MAR-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pC.HA Vav2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7666)
AUTHORS Moores SL, Selfors LM, Fredericks J, Breit T, Fujikawa K, Alt FW,
Brugge JS, Swat W
TITLE Vav family proteins couple to diverse cell surface receptors.
JOURNAL Mol Cell Biol. 2000 Sep . 20(17):6364-73.
PUBMED 10938113
REFERENCE 2 (bases 1 to 7666)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 26, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7666
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 505..529
/label=LNCX
/note="Human CMV promoter, forward primer"
5'UTR 598..637
/label=HSV TK 5'-UTR
/note="5' untranslated region from the herpes simplex virus
thymidine kinase gene"
CDS 3307..3333
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
intron 3365..3937
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind complement(4069..4088)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 4134..4189
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(4188..4207)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
rep_origin 4543..4678
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(4597..4616)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(4784..4801)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4955..5543)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5035..5054)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5714..6574)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6337..6356
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6575..6679)
/gene="bla"
/label=AmpR promoter
primer_bind 6747..6765
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(6803..6825)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 6925..6944
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
rep_origin complement(7011..7466)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7148..7169)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 7360..7379
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7592..7614
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 7606..7623
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 7607..7623
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 7630..7649
/label=T7
/note="T7 promoter, forward primer"
promoter 7630..7648
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgggggat
601 cttggtggcg tgaaactccc gcacctcttc ggccagcgcc ttgtagaagc gcgtatggct
661 tctagaatgg agcagtggcg acagtgcggc cgctggctca tcgattgcaa ggtcctgccg
721 cccaaccacc gggtggtgtg gccctcggcc gtggtcttcg acctggcgca ggcgctgcgc
781 gacggggtcc ttctgtgcca gctgctgcac aacctctccc ccggctccat cgacctcaag
841 gacatcaact tccggccgca gatgtcccag tttctgtgtt tgaagaacat acgcaccttc
901 ctgaaagtct gccacgataa atttggatta aggaacagcg agctgtttga cccctttgac
961 ctcttcgatg tgcgagactt tggaaaggtc atctccgcgg tgtcgaggct ctccctgcac
1021 agcatcgcgc agaacaaagg gatcaggcct tttccctcag aggagaccac agagaatgac
1081 gatgacgtct accgcagcct ggaggagctg gccgacgagc atgacctggg ggaggacatc
1141 tacgactgcg tcccgtgtga ggatggaggg gacgacatct acgaggacat catcaaggtg
1201 gaggtgcagc agcccatgat tagatacatg cagaaaatgg gcatgactga agatgacaag
1261 aggaactgct gcctgctgga gatccaggag accgaggcca agtactaccg caccctggag
1321 gacattgaga agaactacat gagccccctg cggctggtgc tgagcccggc ggacatggca
1381 gctgtcttca ttaacctgga ggacctgatc aaggtgcatc acagcttcct gagggccatc
1441 gacgtgtccg tgatggtggg gggcagcacg ctggccaagg tcttcctcga tttcaaggaa
1501 aggctcctga tctacgggga gtactgcagc cacatggagc acgcccagaa cacactgaac
1561 cagctcctgg ccagccggga ggacttcagg cagaaagtcg aggagtgcac actgaaggtc
1621 caggatggaa aatttaagct gcaagacctg ctggtggtcc ccatgcagag ggtgctcaaa
1681 taccacctgc tcttgaagga gcttctgagc cattctgcgg aacggcctga gaggcagcag
1741 ctcaaagaag cactggaagc catgcaggac ttggcgatgt acatcaatga agttaaacgg
1801 gacaaggaga ccttgaggaa aatcagcgaa tttcagagtt ctatagaaaa tttgcaagtg
1861 aaactggagg aatttggaag accaaagatt gacggggaac tgaaagtccg gtccatagtc
1921 aaccacacca agcaggacag gtacttgttc ctgtttgaca aggtggtcat cgtctgcaag
1981 cggaagggct acagctacga gctcaaggag atcatcgagc tgctgttcca caagatgacc
2041 gacgacccca tgaacaacaa ggacgtcaag aagtctcacg ggaaaatgtg gtcctacggc
2101 ttctacctaa ttcaccttca aggaaagcag ggcttccagt ttttctgcaa aacagaagat
2161 atgaagagga agtggatgga gcagtttgag atggccatgt caaacatcaa gccagacaaa
2221 gccaatgcca accaccacag tttccagatg tacacgtttg acaagaccac caactgcaaa
2281 gcctgcaaaa tgttcctcag gggcaccttc taccagggat acatgtgtac caagtgtggc
2341 gtcggggcac acaaggagtg cctggaagtg atacctccct gcaagttcac ttctcctgca
2401 gatctggacg cctccggagc gggaccaggt cccaagatgg tggccgtgca gaattaccat
2461 ggcaacccag cccctcccgg gaagcctgtg ctgaccttcc agaccggcga cgtgcttgag
2521 ctgctgaggg gcgaccctga gtctccgtgg tgggagggtc gtctggtaca aaccaggaag
2581 tcagggtatt tccccagctc atctgtgaag ccctgccctg tggatggaag gccgcccatc
2641 agccggccgc catcccggga gatcgactac actgcatacc cctggtttgc aggtaacatg
2701 gagaggcagc agacggacaa cctgctcaag tcccacgcca gtgggaccta cctgatcagg
2761 gagcggcctg ccgaggctga gcgctttgca ataagcatca agttcaatga tgaggtgaag
2821 cacatcaagg tggtggagaa ggacaactgg atccacatca cagaggccaa gaaattcgac
2881 agcctcctgg agttggtgga gtactaccag tgccactcac tgaaggagag cttcaagcag
2941 ctggacacca cactcaagta cccctacaag tcccgggaac gttcggcctc cagagcctcc
3001 agccggtccc cagcttcctg tgcttcctac aacttttctt ttctcagtcc tcagggcctc
3061 agctttgctt ctcagggccc ctccgctccc ttctggtcag tgttcacgcc ccgcgtcatc
3121 ggcacagctg tggccaggta taactttgcc gcccgagata tgagggagct ttcgctgcgg
3181 gagggtgacg tggtgaggat ctacagccgc attggcggag accagggctg gtggaagggc
3241 gagaccaacg gacggattgg ctggtttcct tcaacgtacg tagaagagga gggcatccag
3301 actagttatc cgtacgacgt accagactac gcataagaaa agtgaggatc ctgagaactt
3361 cagggtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt
3421 atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca
3481 ccatggaccc tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc
3541 tcctcttatt ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta
3601 acgaattttt aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact
3661 tttttttcaa ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat
3721 tgttataatt aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa
3781 tattcttatt ggtagaaaca actacaccct ggtcatcatc ctgcctttct ctttatggtt
3841 acaatgatat acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc
3901 tgctaaccat gttcatgcct tcttctcttt cctacagctc ctgggcaacg tgctggttgt
3961 tgtgctgtct catcattttg gcaaagaatt cactcctcag gtgcaggctg cctatcagaa
4021 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
4081 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
4141 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
4201 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
4261 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
4321 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
4381 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
4441 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
4501 tctcttatgg agatccctcg aggagctttt tgcaaaagcc taggcctcca aaaaagcctc
4561 ttcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
4621 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
4681 gcggagttag gggcgggact atggttgctg actaattgct gcattaatga atcggccaac
4741 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
4801 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
4861 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
4921 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
4981 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
5041 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
5101 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
5161 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
5221 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
5281 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
5341 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
5401 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
5461 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
5521 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
5581 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
5641 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
5701 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
5761 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
5821 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
5881 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
5941 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
6001 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
6061 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
6121 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
6181 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
6241 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
6301 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
6361 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
6421 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
6481 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
6541 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
6601 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
6661 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
6721 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
6781 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
6841 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
6901 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
6961 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag
7021 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
7081 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
7141 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
7201 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
7261 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
7321 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
7381 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
7441 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa
7501 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
7561 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
7621 agtgaattgt aatacgactc actatagggc gaattcgagc tcgccc
//
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